Properties of urophysial proteins (urophysins) from the white sucker, Catostomus commersoni.

Polyacrylamide gel electrophoresis, polyacrylamide gel electrofocusing and equilibrium dialysis studies on extracts of the urophysis of the white sucker, Catostomus commersoni, have shown the existence of a group of proteins which are specific to the urophysis and which bind two biologically active urophysial peptides, urotensin I (rat hypctensive principle) and urotensin II (fish smooth muscle stimulating principle).     

Increased cathepsin D protein expression is a biomarker for osteosarcomas,pulmonary metastases and other bone malignancies

Cancer proteomics provide a powerful approach to identify biomarkers for personalized medicine. Particularly, biomarkers for early detection, prognosis and therapeutic intervention of bone cancers, especially osteosarcomas, are missing. Initially, we compared two-dimensional gel electrophoresis (2-DE)-based protein expression pattern between cell lines of fetal osteoblasts, osteosarcoma and pulmonary metastasis derived from osteosarcoma. Two independent statistical analyses by means of PDQuest^® and SameSpot^® software revealed a common set of 34 differentially expressed protein spots (p < 0.05). 17 Proteins were identified by mass spectrometry and subjected to Ingenuity Pathway Analysis resulting in one high-ranked network associated with Gene Expression, Cell Death and Cell-To-Cell Signaling and Interaction. Ran/TC4-binding protein (RANBP1) and Cathepsin D (CTSD) were further validated by Western Blot in cell lines while the latter one showed higher expression differences also in cytospins and in clinical samples using tissue microarrays comprising osteosarcomas, metastases, other bone malignancies, and control tissues. The results show that protein expression patterns distinguish fetal osteoblasts from osteosarcomas, pulmonary metastases, and other bone diseases with relevant sensitivities between 55.56% and 100% at ≥87.50% specificity. Particularly, CTSD was validated in clinical material and could thus serve as a new biomarker for bone malignancies and potentially guide individualized treatment regimes.     

全自动毛细管电泳法和琼脂糖凝胶法检测一例多发性骨髓瘤患者血清蛋白结果变化

目的 证明全自动毛细管电泳法比琼脂糖凝胶法更灵敏快捷。方法 分别用毛细管电泳法和琼脂糖凝胶法分析一例多发性骨髓瘤（MM）患者治疗前后血清蛋白。结果 毛细管电泳法治疗前、后，β-球蛋白为41．6％和19，7％；琼脂糖凝胶法治疗前、后，p球蛋白为29.6％和13．3％。结论 全自动毛细管电泳法比琼脂糖凝胶法更灵敏，是血清蛋白电泳有效方法。     

应用脉冲场凝胶电泳技术对浙江省福氏4c型痢疾志贺菌的分型研究

目的 通过应用脉冲场凝胶电泳技术对浙江省痢疾志贺菌福氏4c(F4c)型菌株进行分子流行病学研究,耐药谱分析,为了解本地区菌型的变化提供资料.方法 60株F4c型志贺菌均按GB16002-1995标准鉴定分型并采用kirby-Bauer法检测其对13种抗生素的敏感性.参照美国疾病预防控制中心PulseNet USA的统一方法进行脉冲场凝胶电泳(PFGE)分型并做同源性分析.结果 60株F4c型菌株都存在多重耐药,其中红霉素、利福平的耐药率最高,达100.0%,其次是奈定酸、氨苄西林、多西环素,耐药率均在96.2%以上;丁胺卡那、庆大霉素最敏感,其敏感率均在86.5%以上.脉冲场凝胶电泳分型60株试验菌株共分为24个型,1型36株占60.0%,且所有的暴发型菌株都为1型,2型2株占3.3%,其余22个型各为1株,各占1.7%.结论 F4c型志贺菌是2004年在浙江首次分离到,PFGE分型1型菌株占60.0%,有明显的优势,并且容易引起暴发,其余各型均为少见菌型.此次检测发现其多重耐药现象严重,这可能与浙江省F4c型菌痢病人迅速增多和高度散发有关,应引起临床和疾病控制部门的高度重视.     

小儿肾小球疾病尿蛋白的二维电泳分析及其应用探讨

[目的]建立和优化小儿肾脏疾病尿蛋白研究的方法系统,探讨其在临床上的应用.并比较几种小儿常见的肾小球疾病尿蛋白二维电泳图谱的差异,探求特异性生物标志蛋白.[方法]2003年1月至2004年1月间入住本院儿科系病房的肾小球疾病患儿晨尿标本91份,离心后进行二维电泳分离尿可溶性蛋白,其中原发性肾病综合征(INS,A组)25例,乙肝病毒相关肾炎(B组)16例,狼疮肾炎(LN,C组)5例,IgA肾炎和过敏炎紫癜肾炎(D组)24例,急性链球菌感染后肾小球肾炎(E组)21例.Melanie 3.0软件进行蛋白斑点检测和配比.[结果]尿蛋白二维电泳银染图谱显示各斑点间分离清晰,各组内凝胶在蛋白质斑点位置上具有较好的重复性,适合计算机图像分析.A～E组凝胶的平均蛋白质点数分别为(104±40),(46±22),(38±12),(43±24),(39±19)个,变异率分别为80.3%,58.7%,38.0%,70.4%,58.7%.A组尿蛋白二维电泳银染图谱显示80%出现α1-AGP蛋白斑点,与其他组相比差异具有显著性(P＜0.05).[结论]通过该研究建立了一套分辨率和重复性均较好的双向凝胶电泳尿蛋白分离系统.通过该方法系统分析不同临床类型肾小球疾病患者尿蛋白组分而获得的资料将有助于尿蛋白谱数据库的建立,为肾脏疾病的非侵入性诊断又提供一个新的方法.     

Origin and nature of the proteins of bile. II. A comparative analysis of serum, hepatic lymph and bile proteins in the dog

Abstract. In the dog, as shown before in the human, the major plasma proteins in hepatic bile (albumin, orosomucoid, transferrin and immunoglobulin IgG), with the exception of immunoglobulin IgA, were found to be derived entirely from the plasma. Biliary IgA was shown to have two sources, the plasma and local biosynthesis. These conclusions were based on comparisons on specific radioactivities of different proteins in plasma and bile, after intravenous injection of canine plasma whose proteins had been labelled biosynthetically with tritium. — The transfer of plasma proteins from blood to bile was found to proceed along two parallel pathways, one involving bulk transfer of unmodified plasma, and one based on molecular sieving favouring the transfer of plasma proteins of low molecular weight. — From a comparative study of hepatic lymph and plasma, it was concluded that the molecular sieve process was completed by the time the plasma proteins reached the interstitial fluid. The sieving membrane was therefore assigned anatomically to the vascular walls, which were found to have the properties of an isoporous membrane with a pore radius of 102 Å. No further molecular sieving was found to take place during the crossing of the second barrier between blood and bile, viz. the epithelial sheet separating interstitial fluid from the bile.     

免疫固定电泳技术检测恶性淋巴瘤的应用

[目的]应用免疫固定电泳法，对恶性淋巴瘤（T细胞淋巴瘤，B细胞淋巴瘤）分型及签别诊断。[方法]经Hydrasys琼脂糖凝胶电泳，在γ球蛋白区，呈现深染M蛋白异常区带，再做免疫固定电泳，免疫球蛋白定量，以鉴定恶性淋巴瘤的类别。[结果]选择110例恶性淋巴瘤患者，经血清蛋白电泳检测发现有M蛋白异常区带的有14例，占12％。经免疫同定电泳图谱显示各异，结合Ig定量检测并根据临床病理学诊断确认110例恶性淋巴细胞瘤中，T细胞淋巴瘤61例，占患者总例数的55％；B细胞淋巴瘤13例，占11％；其他类型36例，占32％。[结论]免疫同定电泳检测有助于恶性淋巴瘤的鉴别分型。     

血红蛋白Lepore病--附1例报告及文献复习

目的 探讨血红蛋白Lepore病(Hb Lepore)的诊断、治疗及发病机理。方法 用SDS聚丙烯酰胺凝胶电泳(PH8．7、PH6．2)及Hb肽链分析法检测此病，用皮质激素等治疗，同时给予切脾。结果 病人获得缓解。结论 Hb Lepore是一种在亚太地区罕见、临床表现较重的特殊珠蛋白生成障碍性血红蛋白病。